CHEM-1028High Performance Liquid Chromatography
Liquid chromatography, focusing on the set-up and operation of this type of instrument, will be covered including the preparation of the mobile phase. Columns including normal phase, reverse phase, and size exclusion chromatography will be discussed as well as the impact of the Van Deemter equation on chromatography. Various detectors will be examined with emphasis on their operation and strengths. Injector systems will also be presented. The chromatography will be processed using a modern computerized data system.